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Identificación molecular de enzimas modificantes de aminoglucósidos en cepas de Enterococcus spp. aisladas en hospitales de la Octava Región de Chile / Molecular identification of aminoglycoside-modifying enzymes among strains of Enterococcus spp. isolated in hospitals of the VIII Region of Chile
Sepúlveda A., Marcela; Bello T., Helia; Domínguez Y., Mariana; Mella M., Sergio; Zemelman Z., Raúl; González R., Gerardo.
Afiliação
  • Sepúlveda A., Marcela; Universidad de Concepción. Facultad de Medicina. Departamento de Medicina Interna. Concepción. CL
  • Bello T., Helia; Universidad de Concepción. Facultad de Ciencias Biológicas. Departamento de Microbiología. Grupo de Investigación en Resistencia a Antibióticos. Concepción. CL
  • Domínguez Y., Mariana; Universidad de Concepción. Facultad de Ciencias Biológicas. Departamento de Microbiología. Grupo de Investigación en Resistencia a Antibióticos. Concepción. CL
  • Mella M., Sergio; Universidad de Concepción. Facultad de Medicina. Departamento de Medicina Interna. Concepción. CL
  • Zemelman Z., Raúl; Universidad San Sebastián. Facultad de Ciencias y Tecnología. Concepción. CL
  • González R., Gerardo; Universidad de Concepción. Facultad de Ciencias Biológicas. Departamento de Microbiología. Grupo de Investigación en Resistencia a Antibióticos. Concepción. CL
Rev. méd. Chile ; 135(5): 566-572, mayo 2007. ilus, tab, graf
Article em Es | LILACS | ID: lil-456672
Biblioteca responsável: BR1.1
ABSTRACT

Background:

Infectious diseases produced by Enterococcus spp, must be treated with a synergistic combination between a penicillin and an aminoglycoside. High level resistance to aminoglycosides is a serious therapeutic problem, since it predicts the loss of synergistic activity of this antimicrobial combination.

Aim:

To investigate the presence of genes encoding aminoglycoside-modifying enzymes (AMEs) among strains of Enterococcus spp with high level of resistance to aminoglycosides. Material and

methods:

The genes encoding some of the AMEs were investigated among 305 aminoglycoside-resistant strains of Enterococcus spp isolated in hospitals of the VIII region of Chile, by dot blot hybridization and Polymerase Chain Reaction (PCS).

Results:

High level resistance to some aminoglycosides was observed in 104 strains (34.1 percent) and 93 of these harbored at íeast one of the genes encoding the investigated AMEs. Three genes were detected aac(6)Ie-aph(2")Ia (14.8 percent) encoding for the enzyme AAC(6)Ie-APH(2")Ia (resistance to all aminoglycosides, except streptomycin); aph(3)IIIa (26 percent), and ant(6)la (28.5 percent) encoding for the phosphorylating enzymes APH(3)Ilia (resistance to kanamycin, amikacin and neomycin), and ANT(6)-la (resistance only to streptomycin), respectively. None of the strains harbored the gene ant (4) which encode for the enzyme ANT (4).

Conclusion:

The low frequency of strains harbouring the bifunctional enzyme (<15 percent), conferring an extended resistance profile to aminoglycosides, allows us to propose the empirical use of aminoglycoside-aminocyclitols, associated to a penicillin, in the treatment of serious infections produced by species of enterococci.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Enterococcus / Farmacorresistência Bacteriana / Aminoglicosídeos / Antibacterianos Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Chile Idioma: Es Revista: Rev. méd. Chile Assunto da revista: MEDICINA Ano de publicação: 2007 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Chile
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Enterococcus / Farmacorresistência Bacteriana / Aminoglicosídeos / Antibacterianos Tipo de estudo: Diagnostic_studies Limite: Humans País/Região como assunto: America do sul / Chile Idioma: Es Revista: Rev. méd. Chile Assunto da revista: MEDICINA Ano de publicação: 2007 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Chile