A population genetics study of Anopheles darlingi (Diptera: Culicidae) from Colombia based on random amplified polymorphic DNA-polymerase chain reaction and amplified fragment lenght polymorphism markers
Mem. Inst. Oswaldo Cruz
; 102(3): 255-262, June 2007. mapas, tab
Article
em En
| LILACS
| ID: lil-452500
Biblioteca responsável:
BR1.1
ABSTRACT
The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers (RAPDs) and amplified fragment length polymorphism markers (AFLPs). Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated genetic markers showed the presence of gene flow, suggesting that Colombian An. darlingi populations are in panmixia. Average genetic diversity, estimated from observed heterozygosity, was 0.374 (RAPD) and 0.309 (AFLP). RAPD and AFLP markers showed little evidence of geographic separation between eastern and western populations; however, the F ST values showed high gene flow between the two western populations (RAPD F ST = 0.029; Nm 8.5; AFLP F ST = 0.051; Nm 4.7). According to molecular variance analysis (AMOVA), the genetic distance between populations was significant (RAPDphiST = 0.084; AFLPphiST = 0.229, P < 0.001). The F ST distances and AMOVAs using AFLP loci support the differentiation of the Guyana biogeographic province population from those of the Chocó-Magdalena. In this last region, Chocó and Córdoba populations showed the highest genetic flow.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Variação Genética
/
Genes de Insetos
/
Genética Populacional
/
Anopheles
Limite:
Animals
País/Região como assunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
/
Project document
País de afiliação:
Colômbia
/
Estados Unidos
País de publicação:
Brasil