An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis

Sharry, Sandra; Cabrera Ponce, José Luis; Herrera Estrella, Luis; Rangel Cano, Rosa María; Lede, Silvia; Abedini, Walter

Sharry, Sandra; Cabrera Ponce, José Luis; Herrera Estrella, Luis; Rangel Cano, Rosa María; Lede, Silvia; Abedini, Walter.

Afiliação

Sharry, Sandra; Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro Experimental de Propagación Vegetativa. La Plata. AR
Cabrera Ponce, José Luis; Instituto Politécnico Nacional. Centro de Estudios Avanzados. Departamento de Genética. Unidad Irapuato. Irapuato. MX
Herrera Estrella, Luis; Instituto Politécnico Nacional. Centro de Estudios Avanzados. Departamento de Genética. Unidad Irapuato. Irapuato. MX
Rangel Cano, Rosa María; Instituto Politécnico Nacional. Centro de Estudios Avanzados. Departamento de Genética. Unidad Irapuato. Irapuato. MX
Lede, Silvia; Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro Experimental de Propagación Vegetativa. La Plata. AR
Abedini, Walter; Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro Experimental de Propagación Vegetativa. La Plata. AR

Electron. j. biotechnol ; Electron. j. biotechnol;9(3)June 2006. ilus

Article em En | LILACS | ID: lil-448812

Biblioteca responsável: CL1.1

ABSTRACT

ABSTRACT

A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100 percent conversion of somatic embryos to plants was practically obtained and 100 percent of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Tipo de estudo: Guideline Idioma: En Revista: Electron. j. biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Argentina / México País de publicação: Chile

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