Germline transformation of Aedes fluviatilis (Diptera: Culicidae) with the piggyBac transposable element
Mem. Inst. Oswaldo Cruz
; 101(7): 755-757, Nov. 2006. ilus, tab
Article
em En
| LILACS
| ID: lil-439459
Biblioteca responsável:
BR1.1
ABSTRACT
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Transformação Genética
/
Elementos de DNA Transponíveis
/
Técnicas de Transferência de Genes
/
Aedes
/
Insetos Vetores
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil