Imaging single-channel calcium microdomains by total internal reflection microscopy
Biol. Res
; 37(4): 675-679, 2004. ilus, graf
Article
em En
| LILACS
| ID: lil-437525
Biblioteca responsável:
CL1.1
ABSTRACT
The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Canais de Cálcio
/
Trocador de Sódio e Cálcio
/
Canais Iônicos
Limite:
Animals
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos
País de publicação:
Chile