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Study of the antibody response against Mycobacterium tuberculosis antigens in Warao Amerindian children in Venezuela
Araujo, Z; Waard, J. H. de; Fernández de Larrea, C; López, D; Fandiño, C; Maldonado, A; Hernández, E; Ocaña, Y; Ortega, R; Singh, M; Ottenhoff, Tom H. M; Arend, S. M; Convit, J.
Afiliação
  • Araujo, Z; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Waard, J. H. de; Universidad Central de Venezuela. Instituto de Biomedicina. Laboratorio de Tuberculosis. Caracas. VE
  • Fernández de Larrea, C; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • López, D; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Fandiño, C; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Maldonado, A; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Hernández, E; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Ocaña, Y; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Ortega, R; Universidade Central de Venezuela. Facultad de Medicina. Escuela de Medicina José Maria Vargas. Caracas. VE
  • Singh, M; GBF and Lionex GmbH. DE
  • Ottenhoff, Tom H. M; Leiden University Medical Center. Department of Infectious Diseases, Immunohematology and Blood Transfusion. NL
  • Arend, S. M; Leiden University Medical Center. Department of Infectious Diseases, Immunohematology and Blood Transfusion. NL
  • Convit, J; Universidad Central de Venezuela. Instituto de Biomedicina. Laboratorio de Tuberculosis. Caracas. VE
Mem. Inst. Oswaldo Cruz ; 99(5): 517-524, Aug. 2004. ilus
Article em En | LILACS | ID: lil-386685
Biblioteca responsável: BR1.1
ABSTRACT
This study was aimed at investigating alternate methods for serodiagnosis of tuberculosis (TB), which are needed because bacteriologic diagnosis of childhood TB is difficult. A selection of 80 serum and saliva samples were tested from Warao indigenous children under 15 years of age; 34 high TB suspects (28 positive and 6 negative for the tuberculin skin test, TST) and 46 healthy contact children (32 positive and 14 negative for the TST). Several enzyme-linked immunosorbent assay (ELISA) serological tests were developed to test for Mycobacterium tuberculosis-specific antibodies, including serum IgA, IgG, IgE, and secretory IgA (sIgA) in saliva against 3 specific antigens (PPD, HSP60, 38 kDa). Of these, 2 antigens, PPD and 38 kDa, showed significantly higher reactivity. The sensitivity and specificity of these tests for diagnosis remained limited, between 26.5 percent and 38.2 percent, and 77.4 percent and 97 percent, respectively. Of all the samples studied and combinations realized between all isotypes and antigens combined with 3 isotypes (anti-PPD IgG, IgE, and anti-38kDa sIgA) managed to detect the largest number of patients, showing an improved sensitivity level of 64.7 percent, although specificity levels dropped to 81.8 percent. These results were compared with the Omega diagnostics commercial kit results. The commercial kits showed significantly lower reactivity (sensitivity of 20 percent and 13.33 percent to Myco G and Complex Plus, respectively) and a specificity of 100 percent. This study shows that in indigenous populations of Venezuela, where invasive procedures cannot be used to select samples but evaluation with a chest X-ray for radiological studies is available, the combination of 3 specific isotypes may be a useful tool to increase diagnostic accuracy with pulmonary TB in this population, when used together with clinical and epidemiological criteria.
Assuntos
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tuberculose / Imunoglobulinas / Anticorpos Antibacterianos Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male País/Região como assunto: America do sul / Venezuela Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2004 Tipo de documento: Article / Project document País de afiliação: Alemanha / Holanda / Venezuela País de publicação: Brasil
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tuberculose / Imunoglobulinas / Anticorpos Antibacterianos Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adolescent / Child / Child, preschool / Female / Humans / Infant / Male País/Região como assunto: America do sul / Venezuela Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2004 Tipo de documento: Article / Project document País de afiliação: Alemanha / Holanda / Venezuela País de publicação: Brasil