Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri / Integrons and antimicrobial resistance gene cassettes in Shigella flexneri strains
Rev. méd. Chile
; 131(7): 727-733, jul. 2003.
Article
em Es
| LILACS
| ID: lil-356053
Biblioteca responsável:
CL12.1
ABSTRACT
BACKGROUND:
The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes.AIM:
To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigella flexneri strains. MATERIAL ANDMETHODS:
In vitro susceptibility to 27 antimicrobials was studied in twenty four Shigella flexneri strains isolated from stools. The presence of integrons class 1, 2 and 3 and antimicrobial resistance gene cassettes was investigated by polymerase chain reaction (PCR) using specific primers for each gene.RESULTS:
Most strains were resistant to one of the following antimicrobials ampicillin, sulphonamide, trimethoprim, tetracycline, streptomycin, sulfamethoxazole-trimethoprim or chloramphenicol. Twenty nine percent were simultaneously resistant to all these antimicrobials. Integrons class 1 and 2 were found in 19 strains (79 per cent). Class 3 integrons were not found. Gene cassettes dfrA1 and ant(3")I were associated to integrons class 2 in most strains (15/20, 75 per cent). Genes cat, tetB and blarTEM were detected in 18/24 (75 per cent), 7/24 (29 per cent) and 4/24 (17 per cent) of the strains, respectively and were not associated to any of the studied integrons. Genes that codify enzymes AAC(6')Ib and APH(3')VI were not detected in any strain.CONCLUSIONS:
The high frequency of integrons found in the studied strains, could partly explain the increasing antimicrobial resistance of Shigella flexneri strains, isolated in Chile.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Shigella flexneri
/
Farmacorresistência Bacteriana
/
Integrons
/
Genes Bacterianos
Limite:
Humans
Idioma:
Es
Revista:
Rev. méd. Chile
Assunto da revista:
MEDICINA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Chile