An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Mem. Inst. Oswaldo Cruz
; 98(7): 915-917, Oct. 2003. ilus
Article
em En
| LILACS
| ID: lil-352394
Biblioteca responsável:
BR1.1
RESUMO
Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Toxoplasma
/
Encéfalo
/
Toxoplasmose Animal
/
Cistos
/
Amilopectina
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil