Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil
Braz. j. microbiol
; Braz. j. microbiol;33(3): 274-278, July-Sept. 2002. ilus
Article
em En
| LILACS
| ID: lil-349783
Biblioteca responsável:
BR32.1
RESUMO
This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5 and 3 gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Vulvovaginite
/
Técnicas In Vitro
/
Vacinas
/
Glicoproteínas
/
Infecções por Herpesviridae
/
Herpesvirus Bovino 1
/
Rinotraqueíte Infecciosa Bovina
Tipo de estudo:
Evaluation_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Brasil
/
Holanda
País de publicação:
Brasil