Estandarización y evaluación del ensayo vibriocida modificado / Standardization and evaluation of the modified vibriocidal assay
Rev. cuba. med. trop
; 51(3): 156-159, Sept.-Dec. 1999.
Article
em Es
| LILACS
| ID: lil-333516
Biblioteca responsável:
BR1.1
ABSTRACT
The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Vibrio cholerae
/
Atividade Bactericida do Sangue
Limite:
Animals
/
Humans
Idioma:
Es
Revista:
Rev. cuba. med. trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Cuba