Producción y purificación parcial de la hemolisina principal (Neumolisina) de Streptococcus pneumoniae

Peraza, Gilda T. Toraño; Méndez, Juan C. Vilaseca; Martínez, Isis Tamargo; Monrrás, Miriam Pérez

Producción y purificación parcial de la hemolisina principal (Neumolisina) de Streptococcus pneumoniae / Production and partial purification of the main hemolysin (pneumolysin) of Streptococcus pneumoniae

Peraza, Gilda T. Toraño; Méndez, Juan C. Vilaseca; Martínez, Isis Tamargo; Monrrás, Miriam Pérez.

Afiliação

Peraza, Gilda T. Toraño; Instituto de Medicina Tropical Pedro Kouri. CU
Méndez, Juan C. Vilaseca; Instituto de Medicina Tropical Pedro Kouri. CU
Martínez, Isis Tamargo; Instituto de Medicina Tropical Pedro Kouri. CU
Monrrás, Miriam Pérez; Instituto de Medicina Tropical Pedro Kouri. CU

Rev. cuba. med. trop ; 51(3): 160-165, Sept.-Dec. 1999.

Article em Es | LILACS | ID: lil-333515

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4 of them were determined to be producers of that enzyme. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzyme whose purity level increased by 4.39 with 100 output.

Assuntos

Humanos; Citotoxinas; Streptococcus pneumoniae; Estreptolisinas; Citotoxinas; Estreptolisinas

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Streptococcus pneumoniae / Estreptolisinas / Citotoxinas Limite: Humans Idioma: Es Revista: Rev. cuba. med. trop Assunto da revista: MEDICINA TROPICAL Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Cuba País de publicação: Cuba

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