Identificación de una proteasa neutra en intestino de Boophilus microplus por electroforesis en geles de poliacrilamida copolimerizados con gelatina / Identification of a neutral protease in the intestine of Boophilus microplus with electrophoresis in polyacrylamide gels copolymerized with gelatin
Rev. cuba. med. trop
; 52(3): 165-169, Sept.-Dec. 2000.
Article
em Es
| LILACS
| ID: lil-333477
Biblioteca responsável:
BR1.1
ABSTRACT
A neutral activity of Boophilus microplus in the intestine was identified by electrophoresis in polyacrilamide gel copolymerized with gelatin. The maximum of activity was attained at pH 6.0. The highest specific activity at that pH was obtained with casein substrate. The disappearance of this activity was observed in both substrates after the addition of phenylmethylsulphonyl fluoride in the reaction mixtures. It was very interesting to find out an endopeptidase activity with these characteristics in the intestine, in spite of the fact that the digestive activity in ticks is intracellular at very acid pH, which does not occur in other insects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Endopeptidases
/
Ixodidae
/
Intestinos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Es
Revista:
Rev. cuba. med. trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Cuba