Aspects of immunity for the AMA-1 family of molecules in humans and non-human primates malarias
Mem. Inst. Oswaldo Cruz
; 89(Suppl.2): 67-70, 1994.
Article
em En
| LILACS
| ID: lil-319948
Biblioteca responsável:
BR1.1
ABSTRACT
The apical membrane antigen (AMA-1) family of malaria merozoite proteins is characterised by a high degree of inter-species conservation. Evidence that the protein (PK66/AMA-1) from the simian parasite Plasmodium knowlesi was protective in rhesus monkeys suggested that the 83kDa P. falciparum equivalent (PF83/AMA-1) should be investigated for protective effects in humans. Here we briefly review pertinent comparative data, and describe the use of an eukaryotic full length recombinant PF83/AMA-1 molecule to develop a sensitive ELISA for the determination of serological responses in endemic populations. The assay has revealed surprisingly high levels of humoral response to this quantitatively minor antigen. We also show that PK66/AMA-1 inhibitory mAb's are active against merozoites subsequent to release from schizont-infected red cells, further implicating AMA-1 molecules in red cell invasion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Plasmodium
/
Proteínas de Protozoários
/
Proteínas de Membrana
/
Antígenos de Protozoários
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Holanda
País de publicação:
Brasil