The iron uptake mechanisms of enteroinvasive "Escherichia coli"
Braz. j. microbiol
; Braz. j. microbiol;31(3): 200-5, jul.-set. 2000. ilus, tab
Article
em En
| LILACS
| ID: lil-297398
Biblioteca responsável:
BR32.1
ABSTRACT
Enteroinvasive "Escherichia coli" strains (EIEC) of different serotypes isolated from patients with acute diarrhea were examined for the ability to produce siderophores and iron-regulated outer membrane proteins (IROMP). For iron starvation cultures were grown at 37ºC in LB supplied with 200(micro)M of (alpha)-(alpha)'dypirydil. All strains produced enterobactin and twelve (40(per cent)) produced aerobactin. The strains showed IROMP varying from 67-82 kDa. Proteins were either induced or stimulated by the iron starvation. Differences were observed in the electrophoretic profile among the serotypes, originating 5 electrophoretic profiles. All serotypes expressed proteins of 82kDa (FepA) and 76 kDa (IutA) (except serotype O28acH(-) that did not produce the 76 kDa protein). Several strains (O29H(-), O144H(-), 0152H(-), and O167H(-)) expressed IutA in the outer membrane, in the abscence of aerobactin production. Additionally to well characterized proteins (FepA and IutA), we found two IROMP of unknown function in some serotypes a 71 kDa protein was detected in three profiles and a 67 kDa protein was present in serotype O152H(-). Moreover, two bands (39 and 43 kDa) which were not iron-regulated bound specifically to human lactoferrin
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Sideróforos
/
Diarreia
/
Escherichia coli
/
Ferro
Limite:
Humans
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Brasil
/
Estados Unidos
País de publicação:
Brasil