Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and it's application in an epidemiological survey in Brazil

Madruga, Cláudio R; Marques, Ana Paula C; Araújo, Flábio R; Miguita, M; Carvalho, Cristiano M. E; Araújo, Fábio S; Umaki, Adriana C. S; Crocci, Adalberto J; Queiróz, Rosângela A

Madruga, Cláudio R; Marques, Ana Paula C; Araújo, Flábio R; Miguita, M; Carvalho, Cristiano M. E; Araújo, Fábio S; Umaki, Adriana C. S; Crocci, Adalberto J; Queiróz, Rosângela A.

Afiliação

Madruga, Cláudio R; EMBRAPA.
Marques, Ana Paula C; EMBRAPA.
Araújo, Flábio R; Universidade para o Desenvolvimento do Estado e da Regiäo do Pantanal.
Miguita, M; EMBRAPA.
Carvalho, Cristiano M. E; EMBRAPA.
Araújo, Fábio S; Universidade para o Desenvolvimento do Estado e da Regiäo do Pantanal.
Umaki, Adriana C. S; Universidade Católica Dom Bosco.
Crocci, Adalberto J; Universidade Estadual Paulista. Instituto de Biociências de Botucatu. Departamento de Bioestatística.
Queiróz, Rosângela A; EMBRAPA.

Pesqui. vet. bras ; Pesqui. vet. bras;21(2): 72-6, abr.-jun. 2001. ilus, tab

Article em En | LILACS | ID: lil-290514

Biblioteca responsável: BR68.1

RESUMO

RESUMO

An indirect enzyme-linked immunosorbent assay (ELISA) using a crude antigen was evaluated for its performance to detect Babesia bigemina antibodies. The sensitivity and specificity were 98.0 per cent and 99.0 per cent, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 10 7(subscribe) Babesia bovis organisms. With regard to the comparison of ELISA and indirect fluorescent antibody test (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves' sera. There was a good agreement between results shown by ELISA and IFAT with sera from an enzootically stable area (k=0.61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (k=0.33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalence ranged from 87.7 to 98.9 per cent. Therefore, this ELISA with high sensitivity, specificity and performance similar to IFAT can be employed in serological diagnosis of B. bigemina

Assuntos

Babesia; Ensaio de Imunoadsorção Enzimática; Testes Sorológicos

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Babesia / Ensaio de Imunoadsorção Enzimática / Testes Sorológicos Tipo de estudo: Diagnostic_studies / Risk_factors_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Pesqui. vet. bras Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2001 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

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