Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;33(8): 889-95, Aug. 2000. tab, ilus
Article
em En
| LILACS
| ID: lil-265845
Biblioteca responsável:
BR1.1
RESUMO
The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4 percent casein as compared to 20 percent casein in the control diet. When the experimental group had attained a 20 percent loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5 percent) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Células da Medula Óssea
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Fibronectinas
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Laminina
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Desnutrição Proteico-Calórica
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Matriz Extracelular
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2000
Tipo de documento:
Article
/
Congress and conference
País de afiliação:
Brasil
País de publicação:
Brasil