Use of a cysteine proteinase from Carica candamarcensis as a protective agent during DNA extraction
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;31(9): 1129-32, sept. 1998. ilus
Article
em En
| LILACS
| ID: lil-222960
Biblioteca responsável:
BR1.1
RESUMO
We describe the use of a plant cysteine proteinase isolated from latex of Carica candamarcensis as a protective agent during isolation of bacterial DNA following growth in culture of these cells. Between 100 to 720 units of proteinase (1 µg = 6 units) afforded good DNA protection when incubated with various kinds of microorganisms. Agarose gel electrophoresis showed that the resulting DNA was similar in size to DNA preparations obtained by treatment with proteinase K. The viability of the resulting material was checked by PCR amplification using species-specific primers. After standing at room temperature (25oC) for 35 days, the enzyme lost 10 percent of its initial activity. The enzyme stability and good yield of DNA suggest the use of this proteinase as an alternative to proteinase K
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
DNA
/
Endopeptidase K
/
Substâncias Protetoras
/
Cisteína Proteases
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1998
Tipo de documento:
Article
País de publicação:
Brasil