In situ hybridization and immunoperoxidase for quick detection and identification of leishmania braziliensis in tissue
Parasitol. día
; 20(3/4): 86-90, jul.-dic. 1996. ilus, tab
Article
em En
| LILACS
| ID: lil-202465
Biblioteca responsável:
CL1.1
ABSTRACT
The combined used of in situ hybridization and immunoperoxidase techniques applied to histological sections of the biopsy allowed an increment of the efficiency in the detection of Leishmania diagnosis.The molecular basis for DNA diagnosis is to allow labelled single-stranded species or strain-specific DNA sequences, selected from well characterised reference species, to find and hybridize with homologous DNA from or in, the unknown isolates of parasites. The use of immunoperoxidase assay was useful in disclosing the antigen pathways and revealed amastigotes and antigenic material in various morphological patterns
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Leishmania braziliensis
/
Técnicas Imunoenzimáticas
/
Hibridização In Situ
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Parasitol. día
Assunto da revista:
PARASITOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
Chile