Electrochemical fixation techniques: II. Electrochemical dog body fixation. Histological study
Arch. med. res
; Arch. med. res;27(2): 127-32, 1996. ilus, tab
Article
em En
| LILACS
| ID: lil-200304
Biblioteca responsável:
MX1.1
RESUMEN
This is the first attempt to harden all organs of a body together without excising them. This process was accomplished in bottom-belted, gastrointestinal (GI) or intravenously (IV) catheterized dog cadavers so as to influx en electrolytic solution containing formaldehyde (ESF). The IV influx of ESF was found to be the best perfusion pathway. After 48 h of immersion in ESF, 24 h current time of 17.5 A of current intensity, 24º to 56ºC, we ended up with thoroughly fixed dog cadavers that were wrapped with ethyl alcoholglycerol gauzes and stored in plastic bags at room temperature. Optical microscopy of every sliced tissue showed normal blood vessels, neurons, glial and Purkinje cells and their nuclei of brain and cerebellum, respectively. Cardiac muscle fibers were of normal appearance. Kidney Bowman's capsule and space were found to be normal excepto for vacuolarly degenerated tubules. Small intestine showed normal epithelial cells andcrypts of Lieberkühn. In liver, sinusoids were normally arrayed but showed vacuolar cell degeneration. Herin a method to attain an electrochemical whole body fixation is described
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Fotomicrografia
/
Técnicas Histológicas
/
Fixação de Tecidos
/
Dissecação
/
Eletroquímica
Limite:
Animals
Idioma:
En
Revista:
Arch. med. res
Assunto da revista:
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
México