Isolation and possible composition of glucosylceramides from Paracoccidioides brasiliensis
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;29(11): 1441-4, Nov. 1996. ilus, tab
Article
em En
| LILACS
| ID: lil-187202
Biblioteca responsável:
BR1.1
ABSTRACT
Twelve different species of neutral monohexosyl ceramides (CMHs) and two species of neutral monohexosyl ceramides were isolated form mycelium and yeast forms of Paracoccidioides brasiliensis, respectively, by a combination of ion-exchange chromatography, HPLC, and HPTLC. The glucosylceramides did not react with sera from patients with paracoccidioidomycosis (PCM). Carbohydrate analysis indicated that CMHs contain glucose. Analysis of (1)H-NMR and mass spectrometry data suggest that the structure of the CMHs is Glcpbeta1(Cer (mycelium forms present 12 different ceramides and yeast forms present 2 different ceramides). The composition of the lipid moieties was analyzed by negative fast atom bombardment mass spectrometry. No glycosphingolipid other than glucosylceramide was detected in P. brasiliensis.
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Paracoccidioides
/
Glucosilceramidas
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
/
Congress and conference
País de publicação:
Brasil