Biolistic-mediated gene expression in guinea pigs and cattle tissues in vivo
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;29(10): 1265-7, Oct. 1996. ilus, tab
Article
em En
| LILACS
| ID: lil-186172
Biblioteca responsável:
BR1.1
ABSTRACT
Foreign genes were introduced and expressed in vivo in guinea pigs and cattle utilizing a new hand-held device based on high-pressure helium gas to accelerate DNA-coated microparticles. Guinea pigs were used to evaluate the physical parameters to introduce and express the exogenous DNA. The best conditions were applied to conduct bombardments in cattle. The results showed a high frequency of gene expression in all the bombarded cattle. This procedure could be used to study the immune responses in cattle and in a wide variety of animals through genetic immunization.
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Expressão Gênica
/
Beta-Galactosidase
/
Biolística
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
Brasil