The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague
Mem. Inst. Oswaldo Cruz
; 91(2): 195-8, Mar.-Apr. 1996. tab
Article
em En
| LILACS
| ID: lil-174379
Biblioteca responsável:
BR15.1
RESUMO
Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3µg of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3 per cent w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 112,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Peste
/
Álcool de Polivinil
/
Ensaio de Imunoadsorção Enzimática
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
Brasil