In vitro induced antibody production for the diagnosis of herpes simplex virus infection in immunosuppressed patients
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;29(4): 485-7, Apr. 1996. tab
Article
em En
| LILACS
| ID: lil-163890
Biblioteca responsável:
BR1.1
ABSTRACT
We developed and evaluated a specific test for herpes simplex virus (HSV) infection based on the secretion of HSV-specific antibodies by lymphocytes stimulated in vitro with HSV-1 antigens. The in vitro induced antibody production (IVIAP) test was used for the diagnosis of HSV infection in 43 seropositive selected subjects:
9 healthy subjects (controls), 30 symptomatic patients (26 of them immunocompromised and 4 immunocompetent) and 4 patients with varicella zoster infection. Anti-HSV antibodies were detected by an immune assay using an anti-human IgG peroxidase conjugate. The test showed a sensitivity of 93 per cent (15/16) and specificity of 92 per cent (1/13) which were confirmed by positive culture or clinical and laboratory follow-up. One AIDS patient had a false-negative result and one false-positive result (1/9) was obtained among the healthy subjects. All patients infected with varicella zoster virus were negative to the IVIAP test. The test is rapid, inexpensive, easy to interpret and can be used for the diagnosis of HSV infections, especially in immunocompromised patients.
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
/
Herpes Simples
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1996
Tipo de documento:
Article
País de publicação:
Brasil