Determination of the efficiency of K99-F41 fimbrial antigen vaccine in newborn calves
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;28(6): 651-4, Jun. 1995. tab
Article
em En
| LILACS
| ID: lil-154933
Biblioteca responsável:
BR1.1
RESUMO
Semipurified K99 and F41 fimbrial antigens were used to prepare an oil-emulsified vaccine against bovine enterotoxigenic colibacillosis. Nine Nelore cows about 7 months pregnant were divided into 3 groups (A, B and C) of 3 animals each, which received different doses of vaccine (1,500 HU, 750 HU and 380 HU, respectively) 8 and 2 weeks before delivery, in the neck by the subcutaneous route. As a control (group D), 3 pregnant cows of the same breed were not vaccinated for later challange of their calves. Vaccine efficiency was measured by the serological tests double diffusion and ELISA. Challenge of calves from the vaccinated and from the three control unvaccinated cows was carried out with the virulent Escherichia coli B41 strain (0101), STa+, K99+, F41+). Two of the 3 calves from unvaccinated cows died within 48 h with acute diarrhea. E. coli B4 was recovered as pure culture from their stools. In contrast, none of the calves born from vaccinated cows presented diarrhea. These data suggest that the antibody transfer to calves through colostrum gave full protection aginst the challenge. This semipurified fimbrial vaccine against K99-F41-harboring strains is the first oil-emulsified immunogen prepared in Brazil, which was not only efficient, but also had no adverse effects on vaccinated pregnant cows
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Vacinas Bacterianas
/
Fímbrias Bacterianas
/
Infecções por Escherichia coli
/
Antígenos de Superfície
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1995
Tipo de documento:
Article
País de publicação:
Brasil