Effect of dimethyl sulfoxide on reverse transcriptase activity
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;28(3): 285-90, Mar. 1995. tab, graf
Article
em En
| LILACS
| ID: lil-154692
Biblioteca responsável:
BR1.1
RESUMO
Atlow concentrations, dimethyl sulfoxide (DMSO), stimulated the avian myeloblastosis virus reverse transcriptase activity. About 40 percent stimulation was obtained in the presence of 5 percent (v/v) DMSO, using activated DNA and polyriboadenylic acid (poly(rA)) as templates, and Mg2+ as divalent cation. A similar stimulation by DMSO was observed with Mn2+ for the poly(rA)-dependent reverse transcriptase activity. DMSO at concentrations higher than 15 percent inhibited the reverse transcriptase reactions, independent of the template-primers used. An exception was detected with the 2'-fluoro analog of poly(rA) as template, where an activation of 100 percent was found in the presence of 20 percent DMSO. The stimulation caused by DMSO could be due to a reduction of the apparent Km value for poly(rA) from 9.1 to 3.3 µg/ml
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Dimetil Sulfóxido
/
DNA Polimerase Dirigida por RNA
/
Vírus da Mieloblastose Aviária
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1995
Tipo de documento:
Article
País de publicação:
Brasil