Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;27(3): 737-41, Mar. 1994. graf
Article
em En
| LILACS
| ID: lil-148948
Biblioteca responsável:
BR1.1
RESUMO
Fencamfamine (FCF) is a psychostimulant drug classified as an indirect dopamine agonist. In the present study we evaluated the daily variation in plasma FCF concentration and in striatal dopamine receptors. Adult male Wistar rats (250-300 g) maintained on a 12-h light/12-h dark cycle (lights on at 0700 h) were used. Rats received FCF (10.0 mg/kg, ip) at 0900, 1500, 2100 or 0300 h and blood samples were collected 30 (N = 6) or 60 (N = 6) min after the injections. Plasma FCF was measured by gas chromatography using an electron capture detector. Two-way ANOVA showed significant differences in FCF concentration when blood samples were collected 30 min after the injection, and the highest value was obtained following injection at 2100 h. Moreover, at 1500, 2100 and 0300 h, plasma FCF levels were significantly lower 60 min after injection when compared to the 30-min interval. Two other groups of rats (N = 6) were decapitated at 0900 or 2100 h and the striata were dissected for the binding assays. The Bmax for [3H]-spiroperidol binding to striatal membranes was higher at 2100 h, without changes in affinity constant (Kd). In conclusion, plasma FCF levels and dopamine receptors undergo daily variation, a phenomenon that should be considered to explain the circadian time-dependent effects of FCF
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Receptores Dopaminérgicos
/
Ritmo Circadiano
/
Norbornanos
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1994
Tipo de documento:
Article
País de publicação:
Brasil