Rapid in vitro detection of HIV-1-specific antibody secretion by cells-culture with virus antigens
Mem. Inst. Oswaldo Cruz
; 87(2): 239-47, abr.-jun. 1992. tab, ilus
Article
em En
| LILACS
| ID: lil-116312
Biblioteca responsável:
BR15.1
RESUMO
The present report describes an alternative method for in vitro detection of HIV-1 -specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this technique have showed high sensitivity and specificity since it was capable of detecting HIV-1 infection early after birth. There were neither false-positivity nor false-negativity when blood samples obtained from HIV-1 seronegative asymptomatic individuals, and HIV-1 seropositive adult patients were analized. This rapid, low cost, simple, highly sensitive and specific assay can be extremely useful for early diagnosis of pediatric HIV infection
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Técnicas In Vitro
/
HIV-1
/
Soropositividade para HIV
/
Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Brasil