Dot-dye-immunoassay for the diagnosis of schistosomiasis mansoni
Mem. Inst. Oswaldo Cruz
; 87(2): 187-90, abr.-jun. 1992. tab, ilus
Article
em En
| LILACS
| ID: lil-116303
Biblioteca responsável:
BR15.1
ABSTRACT
A new serological assay dot-dye-immunoassay (dot-DIA) was evaluated for the diagnosis of schistosomiasis mansoni. This method consist of four steps:
(a) biding of antigens to a nitrocellulose membrane (NC); (b) blocking of free sites of the NC; (c) incubation in specific primary antibody; (d) detection of primary antibody reactivity by color development using second antibody coupled to textile dyes. Sera from 82 individuals, 61 with Schistosoma mansoni eggs in the stool and 21 stool negative were tested by ELISA, dot-ELISA, and dotDIA. A high level of agreement between the methods tested was observed for all sera tested ELISA x dot-ELISA 95.1%, ELISA x dot-DIA 92.7% and dot-ELISA x dot-DIA 97.6%. In this study, dot-DIA proved to be a feasible, sensitive, rapid and practical test for the diagnosis of shcistosomiasis
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Esquistossomose mansoni
/
Ensaio de Imunoadsorção Enzimática
/
Imunoensaio
/
Técnicas Imunoenzimáticas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Brasil