Increasing the efficiency of lipid-conjugated antibodies incorporation into the membrane of antigen presenting B cells
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;25(9): 909-12, 1992. ilus
Article
em En
| LILACS
| ID: lil-113591
Biblioteca responsável:
BR26.1
RESUMO
We have introduced some modifications in the technique called "cell decoration" in order to increase the amount of lipid-conjugated antibodies which can be incorporated into the membrane of B cells. As shown by FACS anlysis, we have obtained an approximately 4-fold increment in the amount of specific antibodies incroporated into the cell membrane. The procedure, which consists of successive changes of the medium that contains the lipid-conjugated antibodies, avoided changes on parameters that interfere with cell viability. The proposed modification resulted in an approximately 2-fold enhancement of the ability of decorated B cells to act as antigen presenting cells for specific T hybridomas
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Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Linfócitos B
/
Membrana Celular
/
Lipídeos
/
Anticorpos
/
Antígenos
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Brasil