Viabilidad de Bacillus subtilis auxotrofos en ausencia de sus metabolitos esenciales. / [Viability of Bacillus subtilis auxotrophs in the absence of their essential metabolites]
Rev. argent. microbiol
; Rev. argent. microbiol;22(1): 1-6, 1990 Jan-Mar.
Article
em Es
| BINACIS
| ID: bin-51733
Biblioteca responsável:
AR32.1
ABSTRACT
The effect of tryptophan and uracil starvation on the viability of the transformant B. subtilis BSA 170 trp- ura- and its parent strains Bacillus subtilis PB 168 trp -C and Bacillus subtilis PB 3308 ura- was examined. These studies were performed at the conditions for competence development, during 16 hours. Our results showed that B. subtilis BSA 170 was resistant to tryptophan-less death during all the assay and was also resistant to uracil-less death during three hours. After this time, viability measurements revealed less colony forming units per milliliter, and decrease of the culture absorbances. The uracil-less death required the presence of tryptophan suggesting that protein synthesis is needed. The parental strains exhibit similar behavior. Bacillus subtilis PB 168 was resistant to tryptophan-less death and B. subtilis PB 3308 showed decrease of the viability after uracil starvation comparable to that of the transformant strain.
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Coleções:
06-national
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AR
Base de dados:
BINACIS
Idioma:
Es
Revista:
Rev. argent. microbiol
Ano de publicação:
1990
Tipo de documento:
Article
País de publicação:
Argentina