Comparison of three serological techniques for the diagnosis of bovine herpesvirus type 1: serum neutralization, enzyme-linked immunosorbent assay and indirect immunofluorescence.

Bratanich, A; Sardi, S; Smitsaart, E; Estevez Madero, J; Schudel, A A

Bratanich, A; Sardi, S; Smitsaart, E; Estevez Madero, J; Schudel, A A.

Afiliação

Schudel, A A; Instituto de Virología, INTA Castelar, Buenos Aires, Argentina..

Rev. argent. microbiol ; Rev. argent. microbiol;22(4): 192-8, 1990 Oct-Dec.

Article em En | BINACIS | ID: bin-51558

Biblioteca responsável: AR32.1

ABSTRACT

ABSTRACT

Serum neutralization (SN), indirect immunofluorescence (IFI) and enzyme-linked immunosorbent assay (ELISA) were compared for their sensitivity and specificity to detect bovine serum antibodies to Bovine Herpesvirus type 1 on 105 bovine serum samples. High correlation coefficients among all three techniques were observed, showing a higher sensitivity for the ELISA test. Due to its simplicity and its feasibility to be adopted in less equipped laboratories, the ELISA test is recommended for large scale epizotiological studies, serological diagnosis and detection of reactive animals.

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Coleções: 06-national / AR Base de dados: BINACIS Tipo de estudo: Diagnostic_studies Idioma: En Revista: Rev. argent. microbiol Ano de publicação: 1990 Tipo de documento: Article País de publicação: Argentina

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