Comparative analysis of three methods to assess viability of mammalian cells in culture.

da Costa, A O; de Assis, M C; Marques, E de A; Plotkowski, M C

da Costa, A O; de Assis, M C; Marques, E de A; Plotkowski, M C.

Afiliação

Plotkowski, M C; Department of Microbiology and Immunology, State University of Rio de Janeiro UERJ, Brazil..

Biocell ; Biocell;23(1): 65-72, 1999 Apr.

Article em En | BINACIS | ID: bin-40091

Biblioteca responsável: AR1.1

ABSTRACT

ABSTRACT

Release of lactate dehydrogenase (LDH) from the cytoplasmic compartment, trypan blue exclusion and methylthiazole tetrazolium (MTT) colorimetric assays were compared with regard to their sensitivity in detecting damage of human cultured epithelial cells induced by sodium fluoride or puromycin. LDH assay did not detect any difference between controls and cells treated with either of the two drugs. Cell monolayers treated with 0.3

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Coleções: 06-national / AR Base de dados: BINACIS Idioma: En Revista: Biocell Ano de publicação: 1999 Tipo de documento: Article País de publicação: Argentina

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Coleções: 06-national / AR Base de dados: BINACIS Idioma: En Revista: Biocell Ano de publicação: 1999 Tipo de documento: Article País de publicação: Argentina