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Desarrollo de improntas para el diagnóstico del virus Epstein-Barr por inmunofluorescencia indirecta. / [Development of slides for Epstein-Barr virus diagnosis by indirect immunofluorescence]
Pérez, Germán R; Taborda, Miguel A; Toffi, Alicia; Palonsky, Marcelo; Pagotto, Melina; Gardiol, Daniela N; Giri, Adriana A.
Afiliação
  • Giri, Adriana A; Area Virología, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. gperez@fbioyf.unr..
Medicina (B.Aires) ; 65(4): 315-20, 2005.
Article em Es | BINACIS | ID: bin-38263
Biblioteca responsável: AR2.1
ABSTRACT
Epstein-Barr virus (EBV) is the main oncogenic lymphotropic agent of the Herpesviridae family and is globally distributed. EBV acute infection occurs in young adults producing infectious mononucleosis. Detection of anti-viral capside antigen (VCA) antibodies indicates previous or present EBV infection. Moreover, high titles of anti-VCA antibodies are observed in EBV-associated neoplasic disorders, such as lymphomas in AIDS patients. The objective of this study was the development and optimization of P3HR1 cell slides for the EBV serologic detection by indirect immunofluorescence (IIF) assay. P3HR1 exponential growth culture cells were stimulated with phorbol-12-mirystoil-13-acetate, collected at different time points and used for slide preparation. IIF assay was performed in each slide using an anti-EBV positive serum as primary antibody. An 11
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Coleções: 06-national / AR Base de dados: BINACIS Tipo de estudo: Diagnostic_studies Idioma: Es Revista: Medicina (B.Aires) Ano de publicação: 2005 Tipo de documento: Article País de publicação: Argentina
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Coleções: 06-national / AR Base de dados: BINACIS Tipo de estudo: Diagnostic_studies Idioma: Es Revista: Medicina (B.Aires) Ano de publicação: 2005 Tipo de documento: Article País de publicação: Argentina