Non enzymatic liver cells isolation: citrate-perchlorate technique
Acta physiol. pharmacol. ther. latinoam
; 46(2): 144-8, 1996. ilus
Article
em En
| BINACIS
| ID: bin-22352
Biblioteca responsável:
BR1.1
ABSTRACT
In the present study we describe a non-enzymatic technique for the isolation of rat hepatocytes by perfusion of liver through portal vein. The perfusion media consist of 1 mM sodium percholorate. 5 mM sodium citrate, 10 mM glucose, 129 mM NaCl and 0.1 per cent bovine-serum albumin at pH 7.4. After purification through diatrizoate gradient, electron microscopical studies revealed that most of purified hepatocytes were well preserved and presented a normal ultrastructure, thus correlating with previous biochemical results. The present method enables the recovery of metabolically and morphologically normal hepatocytes. (AU)
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Coleções:
06-national
/
AR
Base de dados:
BINACIS
Assunto principal:
Separação Celular
/
Citratos
/
Compostos de Sódio
/
Percloratos
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Acta physiol. pharmacol. ther. latinoam
Ano de publicação:
1996
Tipo de documento:
Article