In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety. / In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety.
Rev. argent. microbiol
; 47(1): 47-9, 2015 Jan-Mar.
Article
em Es
| BINACIS
| ID: bin-133755
ABSTRACT
The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples. Hence, the purpose of this study was to utilize the insertion sequence IS6110 for amplification of DNA from a smear-positive sample for tuberculosis (TB) diagnosis. Among the 52 positive bacilloscopies, sensitivity, specificity, positive predictive value and negative predictive value were 52.3
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1
Coleções:
01-internacional
/
06-national
/
AR
Base de dados:
BINACIS
/
LILACS
Assunto principal:
Tuberculose
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Observational_studies
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Prognostic_studies
Idioma:
En
/
Es
Revista:
Rev. argent. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Argentina