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Erythrophagocytosis assay in patients with autoimmune hemolytic anemia
Biondi, Claudia; Cotorruelo, Carlos; Garcia Borras, Silvia; Ensinck, Alejandra; Racca, Liliana; Di Monaco, René; Racca, Amelia.
Afiliação
  • Biondi, Claudia; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Inmunología. Argentina
  • Cotorruelo, Carlos; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Inmunología. Argentina
  • Garcia Borras, Silvia; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Inmunología. Argentina
  • Ensinck, Alejandra; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Inmunología. Argentina
  • Racca, Liliana; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Estadística. Argentina
  • Di Monaco, René; Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Argentina
  • Racca, Amelia; Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Area Inmunología. Argentina
Medicina [B.Aires] ; 61(1): 49-52, 2001. tab, gra
Article em En | BINACIS | ID: bin-10517
Biblioteca responsável: BR1.1
ABSTRACT
The aim of this paper is to evaluate the erythrophagocytosis assay (EA) in patients with autoimmune hemolytic anemia (AIHA). Direct antiglobulin test (DAT), indirect antiglobulin test (ITA) and EA were performed in blood samples from 46 patients with presumed AIHA. The EA was carried out incubating patientserythocytes and peripheral blood monocyte. A total of 200 monocytes were analysed to determine the percentage of active phagocytic cells (porcentage APC). In 9 of these patients the applied treatment was evaluated by DAT,IAT and EA. In 14 transfusion requirements, the compatibility tets and EA were performed. For EA, patientsmonocyte were incubated with erytrocytes from previously selected units sensitized with patientssera. The porcentage of APC was 32.1 +/- 1.7 in 35 patients with positive DAT and 17.8 +/- 1.3 in 11 patients with negative DAT. This last value was significantly higher than that with negative controls (3.7 +/- 0.3) (p
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Coleções: 06-national / AR Base de dados: BINACIS Assunto principal: Fagocitose / Eritrócitos / Anemia Hemolítica Autoimune Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Medicina [B.Aires] Ano de publicação: 2001 Tipo de documento: Article
Buscar no Google
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Imprimir
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Coleções: 06-national / AR Base de dados: BINACIS Assunto principal: Fagocitose / Eritrócitos / Anemia Hemolítica Autoimune Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Medicina [B.Aires] Ano de publicação: 2001 Tipo de documento: Article