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Effect of bee venom on IL-6, COX-2 and VEGF levels in polycystic ovarian syndrome induced in Wistar rats by estradiol valerate
Karimzadeh, Latifeh; Nabiuni, Mohammad; Kouchesfehani, Homa Mohseni; Adham, Hamed; Bagheri, Amir; Sheikholeslami, Azar.
Afiliação
  • Karimzadeh, Latifeh; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
  • Nabiuni, Mohammad; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
  • Kouchesfehani, Homa Mohseni; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
  • Adham, Hamed; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
  • Bagheri, Amir; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
  • Sheikholeslami, Azar; Kharazmi University. School of Biological Sciences. Department of Cell and Molecular Biology. Karaj. IR
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;19: 32, maio 2013. graf, ilus
Article em En | LILACS, VETINDEX | ID: biblio-954701
Biblioteca responsável: BR68.1
ABSTRACT

Background:

Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenemia, hirsutism, chronic anovulation and vascular disorder. Interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) are triggered by inflammatory stimuli and lead to angiogenesis and pathogenesis of the ovary. Honeybee venom (HBV) contains an array of biologically active components possessing various pharmaceutical properties. This study was designed to assess the possibility of HBV application as an anti-inflammatory therapeutic agent to suppress levels of the main inflammatory mediators IL-6, COX-2 and VEGF. To induce PCOS, 1 mg of estradiol valerate (EV) per 100 g of body weight was subcutaneously (SC) injected into eight-week-old rats. After 60 days, 0.5 mg/kg of HBV was administered Intraperitoneal (IP) for 14 consecutive days, and the results of PCOS treatment were investigated. Rats were then anesthetized with CO2, and the ovaries were surgically removed. Serum IL-6 was detected by the ELISA kit. Immunoexpression of COX-2 and VEGF were examined in three groups EV-induced PCOS, HBV-treated PCOS and control animals.

Results:

Thickness of theca layer, number and diameter of cysts and levels of IL-6 significantly decreased in HBV group relative to PCOS group. The immunohistochemical analysis showed an increase in COX-2 and VEGF expression in PCOS group whereas HBV-treated rats presented weak and irregular immunostaining.

Conclusions:

Our results suggest that the beneficial effect of HBV may be mediated through its inhibitory effect on serum IL-6 level and ovarian COX-2 and VEGF expression.(AU)
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Ovário / Venenos de Abelha / Interleucina-6 / Ratos Wistar / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS / VETINDEX Assunto principal: Ovário / Venenos de Abelha / Interleucina-6 / Ratos Wistar / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil