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Effect of EDTA on TGF-ß1 released from the dentin matrix and its influence on dental pulp stem cell migration
Gonçalves, Lidiany Freitas; Fernandes, Ana Paula; Cosme-Silva , Leopoldo; Colombo, Fabio Antonio; Martins, Natália Silva; Oliveira, Thais Marchini; Araujo, Tomaz Henrique; Sakai, Vivien Thiemy.
Afiliação
  • Gonçalves, Lidiany Freitas; Universidade Federal de Alfenas. School of Dentistry. Department of Clinics and Surgery. Alfenas. BR
  • Fernandes, Ana Paula; Universidade de São Paulo. Bauru School of Dentistry. Department of Pediatric Dentistry, Orthodontics and Public Health. Bauru. BR
  • Cosme-Silva , Leopoldo; Universidade Federal de Alfenas. School of Dentistry. Department of Clinics and Surgery. Alfenas. BR
  • Colombo, Fabio Antonio; Universidade Federal de Alfenas. Institute of Biomedical Sciences. Department of Pathology and Parasitology. Alfenas. BR
  • Martins, Natália Silva; Universidade Federal de Alfenas. Institute of Exact Sciences. Alfenas. BR
  • Oliveira, Thais Marchini; Universidade de São Paulo. Bauru School of Dentistry. Department of Pediatric Dentistry, Orthodontics and Public Health. Bauru. BR
  • Araujo, Tomaz Henrique; Universidade Federal de Alfenas. Institute of Biomedical Sciences. Department for Cell, Tissue and Developmental Biology. Alfenas. BR
  • Sakai, Vivien Thiemy; Universidade Federal de Alfenas. School of Dentistry. Department of Clinics and Surgery. Alfenas. BR
Braz. oral res. (Online) ; 30(1): e131, 2016. tab, graf
Article em En | LILACS | ID: biblio-951958
Biblioteca responsável: BR1.1
ABSTRACT
Abstract Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Irrigantes do Canal Radicular / Células-Tronco / Movimento Celular / Ácido Edético / Polpa Dentária / Dentina / Fator de Crescimento Transformador beta1 Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Braz. oral res. (Online) Assunto da revista: ODONTOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Irrigantes do Canal Radicular / Células-Tronco / Movimento Celular / Ácido Edético / Polpa Dentária / Dentina / Fator de Crescimento Transformador beta1 Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Braz. oral res. (Online) Assunto da revista: ODONTOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil