Your browser doesn't support javascript.
loading
Production and Characterization of Bacteriocin Produced by Lactobacillus Viridescence(NICM 2167)
KP, Sure; PV, Kotnis; PK, Bhagwat; RC, Ranveer; PB, Dandge; AK, Sahoo.
Afiliação
  • KP, Sure; Shivaji University. Department of Food Science and Technology. IN
  • PV, Kotnis; Shivaji University. Department of Food Science and Technology. IN
  • PK, Bhagwat; Shivaji University. Department of Microbiology. IN
  • RC, Ranveer; Shivaji University. Department of Food Science and Technology. IN
  • PB, Dandge; Shivaji University. Department of Biochemistry. IN
  • AK, Sahoo; Shivaji University. Department of Technology. Maharashtra. IN
Braz. arch. biol. technol ; Braz. arch. biol. technol;59: e16150518, 2016. tab, graf
Article em En | LILACS | ID: biblio-951417
Biblioteca responsável: BR1.1
ABSTRACT
The present study focused on the production optimization of bacteriocin by Lactobacillus viridescence NICM 2167 followed by its purification and characterization. The bacteriocins are antimicrobial peptides produced by many Gram positive and Gram negative bacteria.The bacteriocin produced by LAB (lactic acid bacteria) received attention in recent years due to their potential application as natural preservatives in food. Bacteriocinproduced by Lactobacillus viridescence showed broad range of antimicrobial activity against food borne pathogens. Production parameters were optimized showing highest production of bacteriocinin MRS broth with pH= 7.0 incubated at 37°C for 48 h. Bacteriocin was purified in two steps involving ammonium sulphate precipitation followed by gel filtration using Sephadex G-100. Purified bacteriocin with single band on SDS-PAGE showed molecular weight of 8.3 kDa. This purified bacteriocin was stable over wide range of pH (4-10) as well as temperatures (4°C-121°C) suggesting it as a potent candidate for preservation of various foods.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. arch. biol. technol Assunto da revista: BIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil