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Effect of hyperbaric oxygenation on the expression of glutathione peroxidase 4 and lactoperoxidase genes in the lung of isogenic mice after ischemia/reperfusion injury in the small bowel
Ikejiri, Adauto Tsutomu; Somaio Neto, Frederico; Bertoletto, Paulo Roberto; Chaves, José Carlos; WakateTeruya, Alexandre Key; Kassuya, Cândida Aparecida Leite; Taha, Murched Omar; Fagundes, Djalma José.
Afiliação
  • Ikejiri, Adauto Tsutomu; Universidade Federal da Grande Dourados. Medical School. BR
  • Somaio Neto, Frederico; UFGD. Medical School. Dourados. BR
  • Bertoletto, Paulo Roberto; UFGD. Medical School. Dourados. BR
  • Chaves, José Carlos; UFGD. Medical School. Dourados. BR
  • WakateTeruya, Alexandre Key; UFGD. Medical School. Dourados. BR
  • Kassuya, Cândida Aparecida Leite; UFGD. Medical School. Dourados. BR
  • Taha, Murched Omar; Universidade Federal de São Paulo. Department of Surgery. Division of Surgical Techniques and Experimental Surgery. BR
  • Fagundes, Djalma José; UNIFESP. Department of Surgery. Division of Surgical Techniques and Experimental Surgery. Sao Paulo. BR
Acta cir. bras ; Acta cir. bras;33(5): 462-471, May 2018. tab, graf
Article em En | LILACS | ID: biblio-949341
Biblioteca responsável: BR1.1
ABSTRACT
Abstract

Purpose:

To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR).

Methods:

Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to anesthesia, laparotomy, small bowel ischemia for 60 minutes and reperfusion for 60 minutes; and three groups treated with HBO during ischemia (HBOG + I), during reperfusion (HBOG + R) and during ischemia and reperfusion (HBOG + IR). Studied 84 genes of oxidative stress by the method (RT-qPCR). Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student's t-test p<0.05).

Results:

Gpx4 and Lpo were hiperexpressed on IRG, showing a correlation with these genes with lung oxidative stress. Treated with HBO, there was a significant reduction on genic expression on HBOG+I.

Conclusion:

Hyperbaric oxygenation showed to be associated with decreased expression of these antioxidant genes, suggesting a beneficial effect on the mechanism of pulmonary oxidative stress whenever applied during the ischemia.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Traumatismo por Reperfusão / Estresse Oxidativo / Glutationa Peroxidase / Oxigenoterapia Hiperbárica / Lactoperoxidase / Pulmão Limite: Animals Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Traumatismo por Reperfusão / Estresse Oxidativo / Glutationa Peroxidase / Oxigenoterapia Hiperbárica / Lactoperoxidase / Pulmão Limite: Animals Idioma: En Revista: Acta cir. bras Assunto da revista: Cirurgia Geral / Procedimentos Cir£rgicos Operat¢rios Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil