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Cinética de muerte de linfocitos en diabetes mellitus tipo 1 y su posible asociación con miR-146a y miR-155 / Kinetics of cell death in type 1 diabetes mellitus and their possible association with miR-146a and miR-155
Duarte S, Lissette; Mancilla A, Georthan; García-Díaz, Diego F; Soto I, Néstor; Pérez B, Francisco.
Afiliação
  • Duarte S, Lissette; Universidad de Chile. Facultad de Medicina. Departamento de Nutrición. CL
  • Mancilla A, Georthan; Universidad de Chile. Facultad de Medicina. Departamento de Nutrición. CL
  • García-Díaz, Diego F; Universidad de Chile. Facultad de Medicina. Departamento de Nutrición. CL
  • Soto I, Néstor; Hospital San Borja Arriarán. Unidad de Endocrinología y Diabetes. CL
  • Pérez B, Francisco; Universidad de Chile. Facultad de Medicina. Departamento de Nutrición. CL
Rev. chil. endocrinol. diabetes ; 11(2): 62-68, abr. 2018. graf
Article em Es | LILACS | ID: biblio-914895
Biblioteca responsável: CL1.1
ABSTRACT

Aim:

Analyze mi-146a and miR-155 expression and its correlation with the apoptosis of lymphocytes T in T1D and control patient. Patients and

Methodology:

17 T1D patients (5 children between 8-14 yr and 12 adults between 19-29 yr). Activated and not activated peripheral mononuclear cells were studied were studied. Cellular activation with anti-CD3 and primary culture with interleukyne-2 by 5 days. Apoptosis assays through flow cytometry. miRNA through Taqman probes. Statistical analysis through Kruskal-Wallis and post-hoc Dunn's test.

Results:

Composition of virgin and memory T CD4 cells showed significant differences for stimulus response in control group (p = 0,0004). Increased memory cells count in control group activated by 7 days than basal (p = 0,0047). For early apoptosis differences were observed in days 3 and 7 with and without activation (p = 0,001). AICD apoptosis showed increases in control group after re-stimulation through TCR (p= 0,03). miR-146a expression was lower in recent-onset T1D children vs recent-onset DM1 adults (p = 0,0167).

Conclusion:

This study shows a differential miR-146a expression in T1D children with respect to T1D adult patients, diminished AICD mechanism in T1D and altered CD4+CD45RA-CD45R0+ memory cells generation in T1D adult patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Linfócitos T / Apoptose / Diabetes Mellitus Tipo 1 Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: Es Revista: Rev. chil. endocrinol. diabetes Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2018 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Chile
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google
Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Linfócitos T / Apoptose / Diabetes Mellitus Tipo 1 Tipo de estudo: Risk_factors_studies Limite: Adolescent / Adult / Child / Female / Humans / Male Idioma: Es Revista: Rev. chil. endocrinol. diabetes Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2018 Tipo de documento: Article / Project document País de afiliação: Chile País de publicação: Chile