Tissue preservation and cell culture of Przewalskii´s horse (Equus przewalskii): an endangered species / Preservação de tecidos e cultura de células do cavalo de Przewalski (Equus przewalskii): uma espécie em extinção
Biosci. j. (Online)
; 27(5): 826-829, sept./oct. 2011.
Article
em En
| LILACS
| ID: biblio-911874
Biblioteca responsável:
BR396.4
ABSTRACT
The Przewalskii´s horse or Mongolian wild horse (Equus przewalskii, Poljakov, 1881) is presently the only species of wild horse in existence. Originally from Asia, it is, classified as in extremely high risk of extinction which puts the species in the seriously threatened category. The aim of this work was the preservation of tissues and the development of cell cultures from tissue samples obtained from a Przewalskii´s horse after its death. Biopsies of skin, skeletal and cardiac muscle, and ear cartilage were removed from a recently dead horse, added to Phosphate Buffer Saline (PBS) and refrigerated until processing. Some of the samples were frozen in liquid nitrogen and the other was grown as explants to generate fibroblast cell monolayers. The cell cultures obtained, were subsequently propagated with low passages, and frozen in liquid nitrogen, thus avoiding genetic and phenotypic alterations. The tissues and cell cultures were thawed to ascertain their viability by checking its progressive grow in a flask. It was not possible to obtain cultures from cardiac muscle. A bank of tissues and cells from the single Przewalskii´s horse that existed in Uruguay was generated, and can be used for scientific purposes and for the conservation of the species in the future
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Preservação Biológica
/
Preservação de Tecido
/
Preservação de Amostras de Água
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Espécies em Perigo de Extinção
/
Cavalos
Idioma:
En
Revista:
Biosci. j. (Online)
Assunto da revista:
Agricultura
/
Disciplinas das Cincias Biol¢gicas
/
Pesquisa Interdisciplinar
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Argentina
/
Uruguai
País de publicação:
Brasil