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The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Chen, Y; Yang, S; Peng, Y; Yang, Z.
Afiliação
  • Chen, Y; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Yang, S; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Peng, Y; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
  • Yang, Z; Linyi People's Hospital. Department of Infectious Diseases. Linyi. CN
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;50(11): e6246, 2017. graf
Article em En | LILACS | ID: biblio-888947
Biblioteca responsável: BR1.1
ABSTRACT
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P<0.05). The 3′UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P<0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Receptores de Interleucina-6 / MicroRNAs / Hepatite B / Cirrose Hepática Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Receptores de Interleucina-6 / MicroRNAs / Hepatite B / Cirrose Hepática Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil