Down-regulation of single-stranded DNA-binding protein 1 expression induced by HCMV infection promotes lipid accumulation in cells

Guo, N; Zhang, N; Yan, L; Cao, X; Lv, F; Wang, J; Wang, Y; Cong, H

Guo, N; Zhang, N; Yan, L; Cao, X; Lv, F; Wang, J; Wang, Y; Cong, H.

Afiliação

Guo, N; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Zhang, N; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Yan, L; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Cao, X; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Lv, F; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Wang, J; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Wang, Y; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN
Cong, H; Tianjin Medical University. Tianjin Chest Hospital. Department of Cardiology. Tianjin. CN

Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;50(11): e6389, 2017. tab, graf

Article em En | LILACS | ID: biblio-888946

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. The expression levels of SSBP1 mRNA and protein after HCMV infection were significantly down-regulated. Furthermore, we found that upregulation of SSBP1 inhibited the expression of atherosclerosis-associated LDLR, SCARB, HMGCR, CETP as well as the accumulation of lipids in the cells. The results showed that the inhibition of SSBP1 by HCMV infection promotes lipid accumulation in the cells.

Assuntos

Humanos; Infecções por Citomegalovirus/metabolismo; Proteínas de Ligação a DNA/metabolismo; Células Endoteliais da Veia Umbilical Humana/metabolismo; Células Endoteliais da Veia Umbilical Humana/virologia; Metabolismo dos Lipídeos/fisiologia; Proteínas Mitocondriais/metabolismo; Aterosclerose/metabolismo; Aterosclerose/virologia; Proteínas de Transferência de Ésteres de Colesterol/metabolismo; Colesterol/análise; Proteínas de Ligação a DNA/genética; Regulação para Baixo; Hidroximetilglutaril-CoA Redutases/metabolismo; Metabolismo dos Lipídeos/genética; Proteínas Mitocondriais/genética; Receptores de LDL/metabolismo; Receptores Depuradores Classe B/metabolismo; Fatores de Tempo

Palavras-chave

HCMV; HUVEC; LDLR; Lipid accumulation; SSBP1

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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Infecções por Citomegalovirus / Proteínas Mitocondriais / Proteínas de Ligação a DNA / Metabolismo dos Lipídeos / Células Endoteliais da Veia Umbilical Humana Limite: Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

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