The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps
An. acad. bras. ciênc
; 89(3): 1629-1633, July-Sept. 2017. graf
Article
em En
| LILACS
| ID: biblio-886770
Biblioteca responsável:
BR1.1
ABSTRACT
ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Vespas
/
DNA
/
Endopeptidase K
/
Genitália
Limite:
Animals
Idioma:
En
Revista:
An. acad. bras. ciênc
Assunto da revista:
CIENCIA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil