Detecção molecular de Bacteroides Forsythus em periodontite humana / Molecular detection of Bacteroides Forsythus in human periodontitis
Oral microbiol. Immunol
; 9(3): 154-60, June 1994. ilus, tab
Article
em En
| BBO
| ID: biblio-852778
Biblioteca responsável:
BR97.1
Localização: BR97.1
ABSTRACT
The usefulness of a digoxigenin-labeled genomic DNA probe for the detection of subgingival Bacteroides Forsythus was examined. In addition, the arbitrarily primed polymerase chain reaction (AP-PCR) was used to delineate the genetic diversity of B. Forsythus periodontal isolates. The DNA probe detected 10(3) B. Forsythus cells and yielded a strong signal at 10 (4) cells. It reacted with B. Forsythus ATCC 43037T and 44 clanical isolates and showed no detectable reactivity with 75 strains of 24 other oral microbial species. In comparison to culture, the DNA probe in a dot-blot method demonstrated a sensitivity of 88.8 percent and a specificity of 38.4 percent (accuracy, 72.5 percent). By colony-blotting on primary plates, a sensitivity of 98.1 percent and a specificity of 53.8 percent (accuracy, 82.5 percent) were obtained. B. Forsythus was detected in 449 (73.1 percent) of 614 periodontitis patients. The occurrence of the organism was closely associated with Porphyromonas gingivalis, both species being present in 54.8 percent and absent in 22.2 percent of 270 study samples. AP-PCR identified 24 B. Forsythus genotypes among 27 test straing. This study demonstrated the utility of a non-radioactive genomic probe for direct detection of B. Forsythus in subgingival specimens. The species showed a considerable degree of genetic diversity. DNA analysis may help to determine the role of B. Forsythus in periodontal disease and its mode of transmission among exposed individuals
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Coleções:
06-national
Base de dados:
BBO
Assunto principal:
Doenças Periodontais
/
Infecções por Bacteroides
/
DNA
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Oral microbiol. Immunol
Ano de publicação:
1994
Tipo de documento:
Article