Biological X-ray irradiator characterization for use with small animals and cells

Bruno, A Colello; Mazaro, SJ; Amaral, LL; Rego, EM; Oliveira, HF; Pavoni, JF

Bruno, A Colello; Mazaro, SJ; Amaral, LL; Rego, EM; Oliveira, HF; Pavoni, JF.

Afiliação

Bruno, A Colello; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR
Mazaro, SJ; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR
Amaral, LL; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR
Rego, EM; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR
Oliveira, HF; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR
Pavoni, JF; Universidade de São Paulo. Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto. Serviço de Radioterapia. Ribeirão Preto. BR

Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;50(3): e5848, 2017. tab, graf

Article em En | LILACS | ID: biblio-839264

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

This study presents the characterization of an X-ray irradiator through dosimetric tests, which confirms the actual dose rate that small animals and cells will be exposed to during radiobiological experiments. We evaluated the linearity, consistency, repeatability, and dose distribution in the positions in which the animals or cells are placed during irradiation. In addition, we evaluated the performance of the X-ray tube (voltage and tube operating current), the radiometric survey (leakage radiation) and safety devices. The irradiator default setting was established as 160 kV and 25 mA. Tests showed that the dose rate was linear overtime (R2=1) and remained stable for long (constant) and short (repeatability) intervals between readings. The mean dose rate inside the animal cages was 1.27±0.06 Gy/min with a uniform beam of 95.40% (above the minimum threshold guaranteed by the manufacturer). The mean dose rate inside the cell plates was 0.92±0.19 Gy/min. The dose rate dependence with tube voltage and current presented a quadratic and linear relationship, respectively. There was no observed mechanical failure during evaluation of the irradiator safety devices and the radiometric survey obtained a maximum ambient equivalent dose rate of 0.26 mSv/h, which exempts it from the radiological protection requirements of the International Atomic Energy Agency. The irradiator characterization enables us to perform radiobiological experiments, and assists or even replaces traditional therapy equipment (e.g., linear accelerators) for cells and small animal irradiation, especially in early research stages.

Assuntos

Animais; Doses de Radiação; Radiometria/instrumentação; Calibragem; Desenho de Equipamento; Aceleradores de Partículas; Radiometria/métodos; Raios X

Palavras-chave

Dosimetric characterization; Dosimetry; Radiochromic film; Radiotherapy; X-ray irradiator

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Doses de Radiação / Radiometria Limite: Animals Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2017 Tipo de documento: Article / Project document País de afiliação: Brasil País de publicação: Brasil

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google