Ultrasound as a safe and reliable guidance for subarachnoid puncture in rabbits
Acta cir. bras
; Acta cir. bras;32(1): 14-21, Jan. 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-837670
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions.Methods:
Forty-five adult female rabbits were divided into 3 groups (G) G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0.5% hyperbaric bupivacaine. A needle was inserted into the S1-S2 subarachnoid space guided by ultrasound. The sensitivity and motility of the animals were evaluated for 3 days, after which the animals were sacrificed for removal of lumbar and sacral portions of the spinal cord for histological examination by light microscopy and immunohistochemistry.Results:
All animals had motor function and pain sensitivity on the evaluation period. No animal had complication during or after the procedures.Conclusion:
Ultrasound is a useful and safe method for the correct identification of the subarachnoid space of rabbits.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Punção Espinal
/
Espaço Subaracnóideo
/
Ultrassonografia de Intervenção
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil