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Antifungal activity of Punica granatum linn extracts against Malassezia pachydermatis
Chamilete, Sophia Aparecida Morro; Matsumoto, Leopoldo Sussumu; Delfino, Fernanda; Pirajá, Guilherme Villa; Silva, Regildo Márcio Gonçalves da; Peixoto, Erika Cosendey Toledo de Mello.
Afiliação
  • Chamilete, Sophia Aparecida Morro; Universidade Estadual do Norte do Paraná. Bandeirantes. BR
  • Matsumoto, Leopoldo Sussumu; Universidade Estadual do Norte do Paraná. Bandeirantes. BR
  • Delfino, Fernanda; Médica Veterinária Autônoma. Jaú. BR
  • Pirajá, Guilherme Villa; Clínica Veterinária e Pet Shop Dog e Cia. Marília. BR
  • Silva, Regildo Márcio Gonçalves da; Universidade Estadual Paulista. Assis. BR
  • Peixoto, Erika Cosendey Toledo de Mello; Universidade Estadual do Norte do Paraná. Bandeirantes. BR
Biosci. j. (Online) ; 39: e39098, 2023.
Article em En | LILACS-Express | LILACS | ID: biblio-1567556
Biblioteca responsável: BR396.4
ABSTRACT
Malassezia pachydermatis causes external otitis, often affecting dogs, and control methods for this microorganism have been resistant to synthetic antifungals. Therefore, this study evaluated the antifungal activity of aqueous extracts of Punica granatum Linn (AEP) fruit peel dehydrated (AEPd) and in natura (AEPn) against Malassezia pachydermatis. The M. pachydermatis samples were from the Microbiology Laboratory of the State University of Northern Paraná (UENP), PR, Brazil. The strains were identified and replicated after inoculation in the Sabouraud dextrose medium. Subsequently, the P. granatum extract was obtained through different extraction

methods:

cold, water bath, decoction, and infusion. Each test was run fivefold at 10, 20, 30, 40, and 50% after 10, 20, and 30 minutes. The sensitivity of isolates was determined with the Kirby-Bauer disc diffusion method and indicated by an inhibition zone larger than 15 mm. The results were evaluated with a 2x3x6 factorial study design, ANOVA, and Tukey's test at 5% significance. AEPn showed antifungal activity on M. pachydermatis strains, and AEPd did not present an inhibitory influence at any concentration and time. Extraction by decoction was the most efficient, followed by water bath, cold, and infusion. The extracts at a 50% concentration showed the best results, but all other doses determined an inhibition zone larger than 15 mm. Thus, AEP showed a significant therapeutic potential for controlling M. pachydermatis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Biosci. j. (Online) Assunto da revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Biosci. j. (Online) Assunto da revista: Agricultura / Disciplinas das Ciˆncias Biol¢gicas / Pesquisa Interdisciplinar Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil