Ag85B with c-di-AMP as mucosal adjuvant showed immunotherapeutic effects on persistent Mycobacterium tuberculosis infection in mice

Liang, Xuan; Cui, Ruonan; Li, Xue; Ning, Huanhuan; Kang, Jian; Lu, Yanzhi; Zhou, Shan; Huang, Xinying; Peng, Yujun; Zhang, Jingyao; Li, Shiyun; Ma, Yanling; Bai, Yinlan

Liang, Xuan; Cui, Ruonan; Li, Xue; Ning, Huanhuan; Kang, Jian; Lu, Yanzhi; Zhou, Shan; Huang, Xinying; Peng, Yujun; Zhang, Jingyao; Li, Shiyun; Ma, Yanling; Bai, Yinlan.

Afiliação

Liang, Xuan; College of Life Sciences, Northwest University. Xian. CN
Cui, Ruonan; College of Life Sciences, Northwest University. Xian. CN
Li, Xue; College of Life Sciences, Northwest University. Xian. CN
Ning, Huanhuan; Air Force Medical University. Department of Microbiology and Pathogen Biology. Xian. CN
Kang, Jian; Air Force Medical University. Department of Microbiology and Pathogen Biology. Xian. CN
Lu, Yanzhi; Air Force Medical University. Department of Microbiology and Pathogen Biology. Xian. CN
Zhou, Shan; Xijing Hospital, Air Force Medical University. Department of Clinical Laboratory. Xian. CN
Huang, Xinying; Air Force Medical University. Basic Medical School. Student Brigade. Xian. CN
Peng, Yujun; Air Force Medical University. Basic Medical School. Student Brigade. Xian. CN
Zhang, Jingyao; Air Force Medical University. Basic Medical School. Student Brigade. Xian. CN
Li, Shiyun; Air Force Medical University. Basic Medical School. Student Brigade. Xian. CN
Ma, Yanling; College of Life Sciences, Northwest University. Xian. CN
Bai, Yinlan; Air Force Medical University. Department of Microbiology and Pathogen Biology. Xian. CN

Braz. j. med. biol. res ; 57: e13409, fev.2024. graf

Article em En | LILACS-Express | LILACS | ID: biblio-1564163

Biblioteca responsável: BR1.1

ABSTRACT

ABSTRACT

Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains the leading cause of mortality by a single infectious agent in the world. M. tuberculosis infection could also result in clinical chronic infection, known as latent TB infection (LTBI). Compared to the current limited treatment, several subunit vaccines showed immunotherapeutic effects and were included in clinical trials. In this study, a subunit vaccine of Ag85B with a novel mucosal adjuvant c-di-AMP (Ag85Bc-di-AMP) was delivered intranasally to a persistent M. tuberculosis H37Ra infection mouse model, which also presented the asymptomatic characteristics of LTBI. Compared with Ag85B immunization, Ag85Bc-di-AMP vaccination induced stronger humoral immune responses, significantly higher CD4+ T cells recruitment, enhanced Th1/Th2/Th17 profile response in the lung, decreased pathological lesions of the lung, and reduced M. tuberculosis load in mice. Taken together, Ag85Bc-di-AMP mucosal route immunization provided an immunotherapeutic effect on persistent M. tuberculosis H37Ra infection, and c-di-AMP, as a promising potential mucosal adjuvant, could be further used in therapeutic or prophylactic vaccine strategies for persistent M. tuberculosis infection as well as LTBI.

Palavras-chave

Adjuvant; Ag85B; C-di-AMP; Immunotherapy; Mycobacterium tuberculosis

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google